validated online statistical tools graphpad quickcalcs  (GraphPad Software Inc)

Journal: Frontiers in Plant Science

Article Title: Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill

doi: 10.3389/fpls.2017.01734

Figure Lengend Snippet: (A–D) Rooting performance in Eucalyptus globulus microcuttings derived from donor plants of different ages after sowing. Bars not sharing a letter are significantly different by Tukey test ( P ≤ 0.05). Values are the means of 2 to 4 independent experiments. Data on graphs (C) and (D) considered only rooted cuttings. Lines on top of bars represent standard errors.

Article Snippet: The results were analyzed by ANOVA followed by Tukey test or Student’s t -test, whenever appropriate, using the statistic packages GraphPad Prism 6 and GraphPad QuickCalcs: t- test calculator.

Techniques: Derivative Assay

Journal: Frontiers in Plant Science

Article Title: Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill

doi: 10.3389/fpls.2017.01734

Figure Lengend Snippet: Gene expression of auxin action mechanism-related genes in E. globulus tip cuttings as a function of donor plant age (rooting competence diminishes between 45 and 75 days). Black bars – 45 days; light gray bars – 75 days, dark gray bars – 105 days. (A) Ethylene and cytokinin auxin crosstalk-related genes ERF and ARR1 . (B) Inhibitors of auxin responsive genes TPL and IAA12 . (C) Auxin receptors ABP1 and TIR1 . (D) Auxin transporter genes AUX1 and PIN1 . Expression levels genes were evaluated using qPCR and normalized against H2B and TUA as endogenous control genes. Values represent average of biological triplicates analyzed in technical quadruplicates. Bars sharing the same letters are not different according to Tukey test with P ≤ 0.05. Lines on top of bars represent standard errors.

Article Snippet: The results were analyzed by ANOVA followed by Tukey test or Student’s t -test, whenever appropriate, using the statistic packages GraphPad Prism 6 and GraphPad QuickCalcs: t- test calculator.

Techniques: Gene Expression, Expressing, Control

Journal: Frontiers in Plant Science

Article Title: Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill

doi: 10.3389/fpls.2017.01734

Figure Lengend Snippet: Gene expression of biosynthesis-related genes in E. globulus tip cuttings as a function of donor plant age (rooting competence diminishes between 30 and 60 days). Black bars – 30 days, gray bars – 45 days, white bars – 60 days, diagonal line bars – 75 days. (A) Auxin biosynthesis genes TAA1 and YUC3 during aging in E. globulus . (B) Carbohydrate biosynthesis genes SS3 and SUS1 during aging in E. globulus . Expression levels genes were evaluated using qPCR and normalized against H2B and TUA as endogenous control genes. Values represent average of biological triplicates analyzed in technical quadruplicates. Bars sharing the same letters are not different according to Tukey test with P ≤ 0.05. Lines on top of bars represent standard errors.

Article Snippet: The results were analyzed by ANOVA followed by Tukey test or Student’s t -test, whenever appropriate, using the statistic packages GraphPad Prism 6 and GraphPad QuickCalcs: t- test calculator.

Techniques: Gene Expression, Expressing, Control

Journal: Frontiers in Plant Science

Article Title: Events Associated with Early Age-Related Decline in Adventitious Rooting Competence of Eucalyptus globulus Labill

doi: 10.3389/fpls.2017.01734

Figure Lengend Snippet: Peroxidase activity and auxin concentration in E. globulus tip cuttings as a function of donor plant age (rooting competence diminishes between 30 and 60 days). (A) Peroxidase (POX) activity. (B) Endogenous indol-3-acetic acid (IAA) concentration. Asterisks indicate significant difference according to Student’s t -test: ∗∗ P ≤ 0.01 and ∗∗∗∗ P ≤ 0.0001. Lines on top of bars represent standard errors.

Article Snippet: The results were analyzed by ANOVA followed by Tukey test or Student’s t -test, whenever appropriate, using the statistic packages GraphPad Prism 6 and GraphPad QuickCalcs: t- test calculator.

Techniques: Activity Assay, Concentration Assay